Fig. 3. FTY720-P activates COX-2. (A) HepG2 cells were treated, 30 min before FTY720-P (7.5nM. 2hours), with indomethacin, a COX inhibitor (100 µM). (B) Effect of FTY720-P (7.5nM. 2hours), on the protein expression of COX-2 in presence of a PKC inhibitor (calphostin C; 50 nM, 30min) and ERK inhibitor (PD98059; 50 µM, 30min) Values are normalized to GAPDH using Image lab software. The blot is representative of an experiment repeated 3 times. Values are means ± SEM of at least 3 observations. Bars not sharing a common letter are considered significantly different from each other at p<0.01.